The author(s) shown below used Federal funds provided by the U.S.
Department of Justice and prepared the following final report:
Document Title: Rapid Microfluidic Human Specific DNA
Quantitation
Author: Eugene Tan
Document No.: 236825
Date Received: December 2011
Award Number: 2008-DN-BX-K009

Rapid Microfluidic Human Specific DNA
Quantitation
Eugene Tan
NetBio, 830 Winter Street, Waltham, MA 02451
This project was supported by Grant Number NIJ 2008-DN-BX-K009 awarded by the National Institute of Justice, Office of Justice Programs, US Department of Justice.

Abstract
The goal of this research program was to develop a human-specific DNA quantitation module for incorporation into NetBio’s fully integrated microfluidic instrument and biochip in development. NetBio is applying microfluidic technology to develop an STR analysis instrument that will perform DNA purification, multiplexed PCR amplification, and electrophoretic separation and detection. This fully integrated biochip and instrument will generate STR profiles in 45 minutes without user intervention.

Commercially available STR typing kits allow the effective generation of highly accurate STR profiles, but only when the input DNA template falls within a narrowly optimized range.  The DNA advisory board to the Federal Bureau of Investigation has recommended the use of human-specific DNA quantitation prior to PCR amplification of casework samples as there is the potential for sample contamination from non-human sources including non-human
mammalian, bacterial, and fungal DNA. Accordingly, a fully-integrated microfluidic system for This document is a research report submitted to the U.S. Department of Justice. This report has not been published by the Department. Opinions or points of view expressed are those of the author(s)
and do not necessarily reflect the official position or policies of the U.S. Department of Justice.

Rapid Microfluidic Human Specific DNA Quantitation Final Report
NIJ Award 2008-DN-BX-K009
2
NetBio September 30, 2010
forensic human identification should perform human-specific DNA quantitation in order to determine precisely the amount of DNA template to be subjected to STR amplification.

This research consisted of three elements:

(I) selection and optimization of a DNA quantitation assay,

(II) design and development of instrumentation and microfluidic biochip for DNA quantitation, and

(III) testing of the microfluidic quantitation system using forensic samples. Based on this research, a microfluidic biochip endpoint PCR assays was developed and evaluated based on assay performance (including time to completion, sensitivity, reproducibility, and dynamic range), ease of implementation in a microfluidic format, and cost.

This research represents an important step towards the development of a fully-integrated STR instrument that can be put to widespread use in forensic laboratories

https://www.ojp.gov/pdffiles1/nij/grants/236825.pdf

https://www.sciencedirect.com/science/article/abs/pii/S016816561100...

https://www.researchgate.net/publication/8649333_Microfluidic_chip_...